abstract：:Aggregation of the high-affinity receptor for IgE (Fc eta RI) on the surface of intact or permeabilized rodent mast cells results in tyrosine phosphorylation of phospholipase C-gamma 1 (PLC gamma 1) and PLC gamma 2, and translocation of both isozymes to the particulate fraction. We report here that activation of resid...

journal_title：Cellular signalling

authors： Atkinson TP,Yang Q

更新日期：1996-09-01 00:00:00

abstract：:This review is intended to discuss recent developments in the molecular pharmacology of the alpha 1-adrenergic receptor (alpha 1-AR) subtypes. After a brief historical development, we will focus on the more contemporary issues having to do with this receptor family. Emphasis will be put on recent data regarding the cl...

journal_title：Cellular signalling

authors： Guarino RD,Perez DM,Piascik MT

更新日期：1996-08-01 00:00:00

abstract：:In order to elucidate the molecular events in signal transduction, examination of the interaction between Ras and Raf-1 seems crucial. Many Raf-1 mutants have been investigated in terms of their binding activities to Ras, where only a few Ras mutants have been examined thus far. We have investigated the Raf-1-binding ...

journal_title：Cellular signalling

authors： Hiwasa T,Kasama M,Nakadai T,Sawada T,Sakiyama S

更新日期：1996-08-01 00:00:00

abstract：:Cyclic adenosine monophosphate (cAMP)-dependent protein kinase A(PKA) is considered to be a physiologic modulator of superoxide generation by stimulated neutrophils. Mechanisms of the inhibitory action of PKA are poorly understood. In this study, we investigated effects of cAMP-elevating agents on the phosphorylation ...

journal_title：Cellular signalling

authors： Bengis-Garber C,Gruener N

更新日期：1996-06-01 00:00:00

abstract：:Nitric oxide (NO) is known to regulate redox-sensitive signalling pathways in physiology and pathophysiology. Depending on its concentration, the NO-releasing compound S-nitrosoglutathione (GSNO) causes negative and positive regulation of thymocyte apoptosis. At levels below 0.6 mM, GSNO produces deoxyribonucleic acid...

journal_title：Cellular signalling

authors： Sandau K,Brüne B

更新日期：1996-03-01 00:00:00

abstract：:Quantitative analysis of the proteins which compromise the stimulatory arm of the adenylyl cyclase cascade indicate that the adenylyl cyclase catalytic component is usually the least highly expressed. The effects on both potency of agonist ligands and maximal output resulting from targetted alterations in expression l...

journal_title：Cellular signalling

authors： Milligan G

更新日期：1996-02-01 00:00:00

abstract：:The stimulatory G protein (Gs) mediates activation of adenylylcyclase by a ligand-receptor complex. Gs is heterotrimeric (alpha beta gamma) and activation can be accomplished by dissociation of the alpha-subunit (Gs alpha) from the beta gamma-subunit complex (G beta gamma). Gs alpha is also a substrate for choleragen ...

journal_title：Cellular signalling

authors： Warner DR,Okuya S,Rebois RV

更新日期：1996-01-01 00:00:00

abstract：:Formycin A (1.0 mM) caused a rapid, sustained and rapidly reversible inhibition of effluent radioactivity in rat pancreatic islets prelabelled with myo-[2-3H]inositol and perifused in the presence of 8.3 mM D-glucose. This coincided with a progressive decrease in islet ATP content and transient inhibition of insulin r...

journal_title：Cellular signalling

authors： Ulusoy S,Sener A,Malaisse WJ

更新日期：1995-11-01 00:00:00

abstract：:Differential regulation of PLC-beta 1 and -beta 2 by the G-protein alpha-subunit, G alpha 11, and by G-protein beta gamma-subunits was studied utilizing recombinant PLC-beta 1 and -beta 2. Rat PLC-beta 1 and human PLC-beta 2 were purified after recombinant baculovirus-mediated expression in Sf9 cells. The catalytic pr...

journal_title：Cellular signalling

authors： Paterson A,Boyer JL,Watts VJ,Morris AJ,Price EM,Harden TK

更新日期：1995-09-01 00:00:00

abstract：:Tyrosine phosphorylation of proteins is a mechanism of signalling for different receptors and is important for cell growth and differentiation. Mast cells and basophils are secretory cells that play a role in inflammatory and immediate allergic reactions. The activation/aggregation of different surface receptors on th...

journal_title：Cellular signalling

authors： Hamawy MM,Mergenhagen SE,Siraganian RP

更新日期：1995-08-01 00:00:00

abstract：:We have compared a new commercially available non-radioactive protein kinase C (PKC) activity assay based on the fluorescent [A9,10K11]glycogen synthase1-11 analogue C1-peptide with a classical radioactive assay based on myelin basic protein4-14 (MBP4-14) and other substrates. The C1-peptide had lower affinity for PKC...

journal_title：Cellular signalling

authors： Erdbrügger W,Strohm P,Michel MC

更新日期：1995-08-01 00:00:00

abstract：:The possible involvement of zeta isozyme of protein kinase C (PKC zeta) in phorbol ester-induced signal transduction was investigated in mouse epidermal cells. Western blot analysis of RESOURCE Q column chromatography eluates obtained from 105,000 g supernatants and particulate fractions of epidermal cells was perform...

journal_title：Cellular signalling

authors： Nishikawa K,Yamamoto S,Nagumo H,Maruyama K,Kato R

更新日期：1995-07-01 00:00:00

abstract：:Regulation by unsaturated fatty acids of glucocorticoid-sensitive gene transcription was studied in HeLa cells transiently transfected with a mouse mammary tumour virus-luciferase reporter gene. Arachidonic acid and docosahexaenoic acid by themselves had no effect on basal levels of luciferase expression. However, the...

journal_title：Cellular signalling

authors： Vallette G,Sumida C,Thobie N,Nunez EA

更新日期：1995-05-01 00:00:00

abstract：:We have investigated the role of the hydrolysis of glycosyl-phosphatidylinositol (GPI) as one of the signalling pathways elicited after interaction of epidermal growth factor (EGF) with its specific plasma membrane receptor (EGFR). Endogenous GPI was characterized in both NIH 3T3 mouse fibroblast cells and in EGFR-tra...

journal_title：Cellular signalling

authors： Clemente R,Jones DR,Ochoa P,Romero G,Mato JM,Varela-Nieto I

更新日期：1995-05-01 00:00:00

abstract：:The role of the ras oncogene in the signalling pathway triggered by platelet-derived growth factor BB (PDGF-BB) has been investigated in a cell line which normally differentiates into myotubes. Following the activation of the N-ras oncogene, however, the cells proliferate and form foci. PDGF-BB stimulated the phosphor...

journal_title：Cellular signalling

authors： Zeytinoğlu H,Griffiths SL,Dawson AP,Gibson I

更新日期：1995-03-01 00:00:00

abstract：:We previously showed that protein kinase C (PKC) induces phosphatidylcholine-hydrolysing phospholipase D activation in osteoblast-like MC3T3-E1 cells and that tyrosine kinase is involved in this activation. Wortmannin, a potent inhibitor of phosphatidylinositol 3-kinase, markedly enhanced the formation of choline indu...

journal_title：Cellular signalling

authors： Kozawa O,Suzuki A,Shinoda J,Oiso Y

更新日期：1995-03-01 00:00:00

abstract：:Proliferation of mammalian cells both in vivo and in vitro is dependent upon physiological concentrations of extracellular Ca2+. Growth factor stimulation of quiescent cells at the G0/G1 border usually results in a rapid mobilization of Ca2+ from both intra- and extracellular pools. However, Ca2+ influx is also requir...

journal_title：Cellular signalling

authors： Takuwa N,Zhou W,Takuwa Y

更新日期：1995-02-01 00:00:00

abstract：:Prostaglandin D2 (PGD2) stimulated the formation of choline in a dose-dependent manner in the range between 10 nM and 10 microM. The effect of PGD2 on the formation of inositol phosphates (EC50 was 20 nM) was more potent than that on the formation of choline (EC50 was 0.5 microM). The formation of choline stimulated b...

journal_title：Cellular signalling

authors： Imamura Y,Kozawa O,Suzuki A,Watanabe Y,Saito H,Oiso Y

更新日期：1995-01-01 00:00:00

abstract：:We investigate the mechanisms underlying the intracellular calcium pulse that occurs in response to extracellular adenosine triphosphate (ATP) in osteoclasts. We find that pre-loading of GDP-beta-S abolishes the response in Ca(2+)-free medium, demonstrating an internal release of Ca2+ via a pathway that involves a G p...

journal_title：Cellular signalling

authors： Yu H,Ferrier J

更新日期：1994-11-01 00:00:00

abstract：:Lovastatin was used to study the role of isoprenylated proteins on stimulus-induced increase of cytosolic Ca2+ in retinoic acid-differentiated U937 and HL-60 cells. Preincubation of the cells with lovastatin for 11-24 h reduced the Ca(2+)-influx induced by PAF of FMLP. The maximal decrease was 60% in U937 cells and 40...

journal_title：Cellular signalling

authors： Haag H,Grünberg B,Weber C,Vauti F,Aepfelbacher M,Siess W

更新日期：1994-09-01 00:00:00

abstract：:Multi-step signal transducing events, such as those mediated by G proteins, have been difficult to study in intact cells. We prepared fluorescently labelled G protein subunits, tetramethylrhodamine-alpha o (TMR-alpha o) and TMR-beta gamma, in order to study their subcellular distribution and lateral mobility. Heterotr...

journal_title：Cellular signalling

authors： Kwon G,Axelrod D,Neubig RR

更新日期：1994-08-01 00:00:00

abstract：:Exponentially growing V79 Chinese hamster lung fibroblasts irradiated with 7 Gy X-rays undergo cell cycle arrest in the S and G2 phases. These arrests are released, probably on completion of DNA repair. A premature release occurs after treatment of irradiated cells with caffeine. This release is accompanied by increas...

journal_title：Cellular signalling

authors： Hain J,Jaussi R,Würgler FE

更新日期：1994-07-01 00:00:00

abstract：:The effect of phosphatase inhibitors okadaic acid and calyculin-A on cAMP formation and adrenocorticotropic hormone (ACTH) secretion in AtT-20 corticotrophs was investigated. Both okadaic acid and calyculin-A inhibited dose-dependently the accumulation of cAMP in cells stimulated with pituitary adenylate cyclase activ...

journal_title：Cellular signalling

authors： Koch B,Lutz-Bucher B

更新日期：1994-05-01 00:00:00

abstract：:We have isolated a cDNA from a human liver library which is 2349 base pairs in length and encodes a near-full length seven transmembrane receptor (432 amino acids), 85% homologous to the amino acid sequence for the rat vasoactive intestinal peptide (VIP) receptor. Northern blot analysis identifies a major species at 3...

journal_title：Cellular signalling

authors： Gagnon AW,Aiyar N,Elshourbagy NA

更新日期：1994-03-01 00:00:00

abstract：:Labelling with [3H]glucosamine was used to prepare a transforming growth factor-beta 1 (TGF-beta 1)-sensitive glycosylphosphatidylinositol (GPI) from monolayer cultures of rabbit articular chondrocytes (RAC), which may be involved in control of the cell cycle. The polar headgroup of this glycosylphosphatidylinositol w...

journal_title：Cellular signalling

authors： Vivien D,Bogdanowicz P,Boumediene K,Martiny L,Haye B,Pujol JP

更新日期：1994-02-01 00:00:00

abstract：:Expression of the human alpha 2A-adrenergic receptor gene is induced by cAMP. The present studies were designed to define potential cAMP-responsive enhancer elements (CREs) in the promoter region of this gene. Regions from the 5'-flanking sequences of the gene were placed in a promoterless vector with a chloramphenico...

journal_title：Cellular signalling

authors： Shilo L,Sakaue M,Thomas JM,Philip M,Hoffman BB

更新日期：1994-01-01 00:00:00

abstract：:We have studied the effects of cholinergic agonists on the rates of insulin release and the concentrations of diacylglycerol (DAG) and intracellular free Ca2+ ([Ca2+]i) in the beta-cell line MIN6. Insulin secretion was stimulated by glucose, by glibenclamide and by bombesin. In the presence of glucose, both acetylchol...

journal_title：Cellular signalling

authors： Weng L,Davies M,Ashcroft SJ

更新日期：1993-11-01 00:00:00

abstract：:This review summarizes some recent findings in human sperm which show that progesterone and 17 alpha hydroxyprogesterone are able rapidly (within seconds) to elevate [Ca2+]i and elicit the acrosome reaction (AR) via a non-genomic cell surface receptor. Progesterone promotes a transient elevation in [Ca2+]i which is bl...

journal_title：Cellular signalling

authors： Blackmore PF

更新日期：1993-09-01 00:00:00

abstract：:We investigated the effects of retinoic acid (RA) on the signalling pathways by prostaglandin E2 (PGE2) in osteoblast-like MC3T3-E1 cells. The pretreatment with RA significantly inhibited the formation of inositol phosphates induced by 10 microM PGE2 in a dose-dependent manner in the range between 0.1 nM and 0.1 micro...

journal_title：Cellular signalling

authors： Kotoyori J,Tokuda H,Oiso Y,Kozawa O

更新日期：1993-07-01 00:00:00

abstract：:Protein kinase C and the annexins appear to share some unusual and potentially important membrane- and calcium-binding properties. While these proteins are calcium response elements, they are not calcium-binding proteins in the formal sense; at intracellular calcium concentrations, they only bind significant amounts o...

journal_title：Cellular signalling

authors： Bazzi MD,Nelsestuen GL

更新日期：1993-07-01 00:00:00

abstract：:The earliest substrates to the transmembrane insulin receptor tyrosine kinase, that would function in insulin signalling, are likely to be associated with the plasma membrane. Rat liver plasma membrane 180,000 M(r) protein (p180) is a substrate to the insulin receptor in vitro [Goren et al. (1990) Cellular Signalling ...

journal_title：Cellular signalling

authors： Goren HJ,Boland D,Fei Q

更新日期：1993-05-01 00:00:00

abstract：:Treatment of lucerne suspension culture cells with glycoprotein elicitor from the phytopathogenic fungus Verticillium albo-atrum R & B triggers Ca(2+)-mediated induction of antimicrobial secondary metabolites termed phytoalexins. The present study investigated the possible role of polyphosphoinositide signal transduct...

journal_title：Cellular signalling

authors： Walton TJ,Cooke CJ,Newton RP,Smith CJ

更新日期：1993-05-01 00:00:00

abstract：:The functional consequence of cyclic AMP-dependent phosphorylation of rap1B for stimulus-induced platelet activation is not known. Platelets were pretreated with the stable prostacyclin-analogue iloprost and resuspended in plasma without iloprost. Western blot analysis showed that rap1B was completely converted into i...

journal_title：Cellular signalling

authors： Siess W,Grünberg B

更新日期：1993-03-01 00:00:00

abstract：:Ethanol, in millimolar concentrations, significantly modifies different transductive systems in human lymphocyte cultures. In particular, the presence of alcohol in the medium more than doubles the [Ca2+]i (from 70-90 to 200-250 nM), increasing Ca2+ fluxes from outside, and inhibits the active transport carried out by...

journal_title：Cellular signalling

authors： Fanò G,Belia S,Mariggiò MA,Antonica A,Agea E,Spinozzi F

更新日期：1993-03-01 00:00:00

abstract：:Beta-adrenergic, alpha-1-adrenergic and glucagon stimulation of glucose release were compared between hepatocytes which were freshly isolated, incubated for 3 h in suspension or cultivated for 4 or 24 h in plastic culture flasks in the presence and absence of the protein kinase C activator 12-O-tetradecanoylphorbol-13...

journal_title：Cellular signalling

authors： Hermsdorf T,Dettmer D

更新日期：1992-11-01 00:00:00

abstract：:In this study we investigated the release of Ca2+ in brain microsomes after Ca2+ loading by the Ca(2+)-ATPase or by the Na+/Ca2+ exchanger. The results show that in microsomes loaded with Ca2+ by the Ca(2+)-ATPase, Ins(1,4,5)P3 (5 microM) released 21 +/- 2% of the total Ca2+ accumulated, and that in the microsomes loa...

journal_title：Cellular signalling

authors： Cristóvão AJ,Carvalho CA

更新日期：1992-11-01 00:00:00

abstract：:Adenosine and its analogues inhibited increases in divalent cation influx stimulated by platelet-activating factor (PAF) and formyl-methionyl-leucyl-phenylalanine (FMLP) in a dose-dependent fashion. This effect was antagonized by theophylline, an adenosine receptor antagonist. When extracellular adenosine was removed ...

journal_title：Cellular signalling

authors： Tsuruta S,Ito S,Mikawa H

更新日期：1992-09-01 00:00:00

abstract：:The stimulatory effect of Mn2+ (1.5-fold), forskolin (1.6-fold) and low (1 microM) concentrations of GTP (1.9-fold) on the adenylyl cyclase of adipocyte membranes from obese, diabetic CBA/Ca mice was markedly enhanced compared to that seen using membranes prepared from their lean littermates. In contrast, receptor-med...

journal_title：Cellular signalling

authors： Palmer TM,Taberner PV,Houslay MD

更新日期：1992-07-01 00:00:00

abstract：:We examined the involvement of cAMP-dependent protein kinase (A kinase)2 in the inhibition by cilostamide, a specific inhibitor of the low Km cAMP-phosphodiesterase (PDE), on 9,11-epithio-11,12-methanothromboxane A2 (STA2)-induced platelet aggregation. For comparative purposes, the PGE1 analogue, 17S-20-dimethyl-trans...

journal_title：Cellular signalling

authors： Nishikawa M,Komada F,Morita K,Deguchi K,Shirakawa S

更新日期：1992-07-01 00:00:00

abstract：:Measurements of prostaglandin E2 (PGE2)-induced adenylyl cyclase activity in membranes isolated from epididymal rat adipocytes revealed inhibition of cAMP production at low concentrations of PGE2 (less than 10 mM) and stimulation at higher concentrations. This biphasic effect of PGE2 was obtained when adenylyl cyclase...

journal_title：Cellular signalling

authors： Cohen-Luria R,Rimon G

更新日期：1992-05-01 00:00:00